Vertical Laminar Flow Hood Aseptic Technique

The direction of air flow may be horizontal or vertical.

Vertical laminar flow hood aseptic technique.

Development maintenance of skills needed to properly use laminar airflow workbench lafw or laminar airflow hood. The air flowing out from the hood suspends and removes contaminants introduced into the work area by personnel. What is the maximum number of particles in a clean room buffer room and ante room. Room air in red enters the system from above the hepa filter.

Cytotoxic agents radioactive agents antimicrobial agents. Use strict aseptic technique to avoid introduction of contaminants into the hood. Turbulence patterns around objects in the vertical flow hood will be different from that in horizontal flow units. Items not sterile including fingers and hands must be kept.

While laminar airflow hoods prevent airborne contamination they do not guarantee a sterile product. Vertical laminar flow hoods are often chosen because they resemble on a small scale the design of a laminar flow cleanroom in which fan filter units are typically positioned in the ceiling. Horizontal flow hoods are most commonly used with the more costly vertical flow hoods being reserved for agents that may produce an environmental hazard e g. Use precautions to minimize contamination in the product preparation area.

Laminar flow hoods are used to control airborne contamination of sterile products during their extemporaneous preparation. Provide clean air to the working area. Supplies may be arranged to the sides and the back of the area in which manipulations will be performed. Provide a constant flow of air out of the work area to prevent room air from entering.

99 99 particle free air is forced downward toward the work surface. Manipulative technique is also different in a vertical flow hood. Vertical laminar flow hoods.